Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization

Jon S. Thorson, William A. Barton, Dirk Hoffmeister, Christoph Albermann, Dimitar B. Nikolov

Producción científica: Review articlerevisión exhaustiva

76 Citas (Scopus)

Resumen

Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

Idioma originalEnglish
Páginas (desde-hasta)16-25
Número de páginas10
PublicaciónChemBioChem
Volumen5
N.º1
DOI
EstadoPublished - ene 3 2004

Financiación

FinanciadoresNúmero del financiador
National Institute of Allergy and Infectious DiseasesR01AI052218

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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