Structure-based profiling of metabolites and isotopomers by NMR

Teresa W.M. Fan; Andrew N. Lane

doi:10.1016/j.pnmrs.2007.03.002

Structure-based profiling of metabolites and isotopomers by NMR

Producción científica: Review article › revisión exhaustiva

220 Citas (Scopus)

Resumen

Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

Idioma original	English
Páginas (desde-hasta)	69-117
Número de páginas	49
Publicación	Progress in Nuclear Magnetic Resonance Spectroscopy
Volumen	52
N.º	2-3
DOI	https://doi.org/10.1016/j.pnmrs.2007.03.002
Estado	Published - feb 4 2008

Nota bibliográfica

Funding Information:
This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.

Financiación

This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.

Financiadores	Número del financiador
Kentucky Challenge for Excellence
National Institutes of Health (NIH)	RR018733
National Childhood Cancer Registry – National Cancer Institute	EPS-0447479, 1 R01 CA101199-01
National Center for Research Resources
Susan G Komen Foundation	BCTR0503648
Kentucky Science and Engineering Foundation	KSEF-148-502-03-51

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Nuclear and High Energy Physics
Spectroscopy

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10.1016/j.pnmrs.2007.03.002

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title = "Structure-based profiling of metabolites and isotopomers by NMR",

abstract = "Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.",

keywords = "2-D NMR, Isotopomer profiling, Metabolic flux, Metabolic pathways, Metabolite profiling, Stable isotopes",

author = "Fan, \{Teresa W.M.\} and Lane, \{Andrew N.\}",

note = "Funding Information: This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science \& Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky. ",

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AU - Lane, Andrew N.

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N2 - Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

AB - Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

KW - 2-D NMR

KW - Isotopomer profiling

KW - Metabolic flux

KW - Metabolic pathways

KW - Metabolite profiling

KW - Stable isotopes

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DO - 10.1016/j.pnmrs.2007.03.002

M3 - Review article

AN - SCOPUS:38749110011

SN - 0079-6565

VL - 52

SP - 69

EP - 117

JO - Progress in Nuclear Magnetic Resonance Spectroscopy

JF - Progress in Nuclear Magnetic Resonance Spectroscopy

IS - 2-3

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Structure-based profiling of metabolites and isotopomers by NMR

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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