[18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

Kiran Kumar Solingapuram Sai; Xiaofei Chen; Zhe Li; Caigang Zhu; Kirtikar Shukla; Tom E. Forshaw; Hanzhi Wu; Stephen A. Vance; Buddhika Liyana Pathirannahel; Megan Madonna; Mark W. Dewhirst; Allen W. Tsang; Leslie B. Poole; Nimmi Ramanujam; S. Bruce King; Cristina M. Furdui

doi:10.1016/j.redox.2021.102218

[¹⁸F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

Kiran Kumar Solingapuram Sai
, Xiaofei Chen
, Zhe Li
, Caigang Zhu
, Kirtikar Shukla
, Tom E. Forshaw
, Hanzhi Wu
, Stephen A. Vance
, Buddhika Liyana Pathirannahel
, Megan Madonna
, Mark W. Dewhirst
, Allen W. Tsang
, Leslie B. Poole
, Nimmi Ramanujam
, S. Bruce King
, Cristina M. Furdui

Producción científica: Article › revisión exhaustiva

5 Citas (Scopus)

Resumen

Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [¹⁸F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.

Idioma original	English
Número de artículo	102218
Publicación	Redox Biology
Volumen	49
DOI	https://doi.org/10.1016/j.redox.2021.102218
Estado	Published - feb 2022

Nota bibliográfica

Publisher Copyright:
© 2021

Financiación

The authors acknowledge financial support for these studies provided by the Center for Redox Biology and Medicine at Wake Forest School of Medicine (pilot funds to S.B.K.), the Wake Forest Baptist Comprehensive Cancer Center (NIH/NCI P30 CA12197 ; pilot funds to C.M.F. and support of shared resources facilities), NIH/NCI R42 CA156901 to N.R., and NIH/NIGMS R35 GM135179 to L.B.P..

Financiadores	Número del financiador
Center for Redox Biology and Medicine at Wake Forest School of Medicine
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	R42 CA156901, P30 CA12197
National Institute of General Medical Sciences DP2GM119177 Sophie Dumont National Institute of General Medical Sciences	R35GM135179
Comprehensive Cancer Center at Wake Forest Baptist Medical Center

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Organic Chemistry

Acceder al documento

10.1016/j.redox.2021.102218

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Solingapuram Sai, K. K., Chen, X., Li, Z., Zhu, C., Shukla, K., Forshaw, T. E., Wu, H., Vance, S. A., Pathirannahel, B. L., Madonna, M., Dewhirst, M. W., Tsang, A. W., Poole, L. B., Ramanujam, N., King, S. B., & Furdui, C. M. (2022). [¹⁸F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo. Redox Biology, 49, Artículo 102218. https://doi.org/10.1016/j.redox.2021.102218

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title = "[18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo",

abstract = "Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [18F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.",

keywords = "Head and Neck Squamous Cell Cancer, PET imaging, Protein sulfenylation, Radiation resistance, [18F]F-DCP",

author = "\{Solingapuram Sai\}, \{Kiran Kumar\} and Xiaofei Chen and Zhe Li and Caigang Zhu and Kirtikar Shukla and Forshaw, \{Tom E.\} and Hanzhi Wu and Vance, \{Stephen A.\} and Pathirannahel, \{Buddhika Liyana\} and Megan Madonna and Dewhirst, \{Mark W.\} and Tsang, \{Allen W.\} and Poole, \{Leslie B.\} and Nimmi Ramanujam and King, \{S. Bruce\} and Furdui, \{Cristina M.\}",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2022",

month = feb,

doi = "10.1016/j.redox.2021.102218",

language = "English",

volume = "49",

}

Solingapuram Sai, KK, Chen, X, Li, Z, Zhu, C, Shukla, K, Forshaw, TE, Wu, H, Vance, SA, Pathirannahel, BL, Madonna, M, Dewhirst, MW, Tsang, AW, Poole, LB, Ramanujam, N, King, SB & Furdui, CM 2022, '[¹⁸F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo', Redox Biology, vol. 49, 102218. https://doi.org/10.1016/j.redox.2021.102218

TY - JOUR

T1 - [18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

AU - Solingapuram Sai, Kiran Kumar

AU - Chen, Xiaofei

AU - Li, Zhe

AU - Zhu, Caigang

AU - Shukla, Kirtikar

AU - Forshaw, Tom E.

AU - Wu, Hanzhi

AU - Vance, Stephen A.

AU - Pathirannahel, Buddhika Liyana

AU - Madonna, Megan

AU - Dewhirst, Mark W.

AU - Tsang, Allen W.

AU - Poole, Leslie B.

AU - Ramanujam, Nimmi

AU - King, S. Bruce

AU - Furdui, Cristina M.

PY - 2022/2

Y1 - 2022/2

N2 - Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [18F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.

AB - Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [18F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.

KW - Head and Neck Squamous Cell Cancer

KW - PET imaging

KW - Protein sulfenylation

KW - Radiation resistance

KW - [18F]F-DCP

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