The alpha-fetoprotein promoter is the target of Afr1-mediated postnatal repression

David K. Peyton; Mei Chuan Huang; Meredith A. Giglia; Nedda K. Hughes; Brett T. Spear

doi:10.1006/geno.1999.6073

The alpha-fetoprotein promoter is the target of Afr1-mediated postnatal repression

David K. Peyton, Mei Chuan Huang, Meredith A. Giglia, Nedda K. Hughes, Brett T. Spear

Producción científica: Article › revisión exhaustiva

23 Citas (Scopus)

Resumen

The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15. (C) 2000 Academic Press.

Idioma original	English
Páginas (desde-hasta)	173-180
Número de páginas	8
Publicación	Genomics
Volumen	63
N.º	2
DOI	https://doi.org/10.1006/geno.1999.6073
Estado	Published - ene 15 2000

Nota bibliográfica

Funding Information:
We thank Marisa Bartolemei for generously providing the H19 probe, Michelle Glenn, Leila Ghabrial, and Carrie Blaydes for technical assistance, and Martha Peterson for critically reading the manuscript. This research is supported by Public Health Service Grants GM45253 and DK51600.

Financiación

We thank Marisa Bartolemei for generously providing the H19 probe, Michelle Glenn, Leila Ghabrial, and Carrie Blaydes for technical assistance, and Martha Peterson for critically reading the manuscript. This research is supported by Public Health Service Grants GM45253 and DK51600.

Financiadores	Número del financiador
National Institute of Diabetes and Digestive and Kidney Diseases	R01DK051600
U.S. Public Health Service	GM45253

ASJC Scopus subject areas

Genetics

Acceder al documento

10.1006/geno.1999.6073

Otros archivos y enlaces

Citar esto

@article{0f47b015ccfc42e88a2bc46018b0e219,

title = "The alpha-fetoprotein promoter is the target of Afr1-mediated postnatal repression",

abstract = "The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15. (C) 2000 Academic Press.",

author = "Peyton, \{David K.\} and Huang, \{Mei Chuan\} and Giglia, \{Meredith A.\} and Hughes, \{Nedda K.\} and Spear, \{Brett T.\}",

note = "Funding Information: We thank Marisa Bartolemei for generously providing the H19 probe, Michelle Glenn, Leila Ghabrial, and Carrie Blaydes for technical assistance, and Martha Peterson for critically reading the manuscript. This research is supported by Public Health Service Grants GM45253 and DK51600.",

year = "2000",

month = jan,

day = "15",

doi = "10.1006/geno.1999.6073",

language = "English",

volume = "63",

pages = "173--180",

number = "2",

}

TY - JOUR

T1 - The alpha-fetoprotein promoter is the target of Afr1-mediated postnatal repression

AU - Peyton, David K.

AU - Huang, Mei Chuan

AU - Giglia, Meredith A.

AU - Hughes, Nedda K.

AU - Spear, Brett T.

N1 - Funding Information: We thank Marisa Bartolemei for generously providing the H19 probe, Michelle Glenn, Leila Ghabrial, and Carrie Blaydes for technical assistance, and Martha Peterson for critically reading the manuscript. This research is supported by Public Health Service Grants GM45253 and DK51600.

PY - 2000/1/15

Y1 - 2000/1/15

N2 - The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15. (C) 2000 Academic Press.

AB - The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034007475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034007475&partnerID=8YFLogxK

U2 - 10.1006/geno.1999.6073

DO - 10.1006/geno.1999.6073

M3 - Article

C2 - 10673330

AN - SCOPUS:0034007475

SN - 0888-7543

VL - 63

SP - 173

EP - 180

JO - Genomics

JF - Genomics

IS - 2

ER -

The alpha-fetoprotein promoter is the target of Afr1-mediated postnatal repression

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto