The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Stephen A. Bell; Arthur G. Hunt

doi:10.1016/j.febslet.2010.03.007

The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Stephen A. Bell
, Arthur G. Hunt

Producción científica: Article › revisión exhaustiva

16 Citas (Scopus)

Resumen

The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.

Idioma original	English
Páginas (desde-hasta)	1449-1454
Número de páginas	6
Publicación	FEBS Letters
Volumen	584
N.º	8
DOI	https://doi.org/10.1016/j.febslet.2010.03.007
Estado	Published - abr 2010

Nota bibliográfica

Funding Information:
The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

Financiación

The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

Financiadores	Número del financiador
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	0817818

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2010.03.007

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title = "The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein",

abstract = "The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.",

keywords = "CPSF30, CstF77, Polyadenylation, RNA-binding",

author = "Bell, \{Stephen A.\} and Hunt, \{Arthur G.\}",

note = "Funding Information: The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.",

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AU - Bell, Stephen A.

AU - Hunt, Arthur G.

N1 - Funding Information: The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

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AB - The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.

KW - CPSF30

KW - CstF77

KW - Polyadenylation

KW - RNA-binding

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DO - 10.1016/j.febslet.2010.03.007

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SN - 0014-5793

VL - 584

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JO - FEBS Letters

JF - FEBS Letters

IS - 8

ER -

The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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