The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Thomas Haarmann; Caroline Machado; Yvonne Lübbe; Telmo Correia; Christopher L. Schardl; Daniel G. Panaccione; Paul Tudzynski

doi:10.1016/j.phytochem.2005.04.011

The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Thomas Haarmann
, Caroline Machado
, Yvonne Lübbe
, Telmo Correia
, Christopher L. Schardl
, Daniel G. Panaccione
, Paul Tudzynski

Plant Pathology

Producción científica: Article › revisión exhaustiva

117 Citas (Scopus)

Resumen

The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

Idioma original	English
Páginas (desde-hasta)	1312-1320
Número de páginas	9
Publicación	Phytochemistry
Volumen	66
N.º	11 SPEC. ISS.
DOI	https://doi.org/10.1016/j.phytochem.2005.04.011
Estado	Published - jun 2005

Nota bibliográfica

Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

Financiación

This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

Financiadores	Número del financiador
C.L.S.
U.S. Department of Agriculture	58-6401-2-0025, 2001-35319-10930
Deutsche Forschungsgemeinschaft	Tu 50/13
Sociedade Portuguesa de Pediatria

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/j.phytochem.2005.04.011

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@article{db95b0b5a1524478a688aee05500de1a,

title = "The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution",

abstract = "The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., H{\"o}lter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.",

keywords = "Chemical races, Claviceps purpurea, Ergocristine, Ergot alkaloids, Ergot fungus, Ergotamine, Gene cluster, Nonribosomal peptide synthetases, Phosphate effect",

author = "Thomas Haarmann and Caroline Machado and Yvonne L{\"u}bbe and Telmo Correia and Schardl, \{Christopher L.\} and Panaccione, \{Daniel G.\} and Paul Tudzynski",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data. ",

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doi = "10.1016/j.phytochem.2005.04.011",

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AU - Haarmann, Thomas

AU - Machado, Caroline

AU - Lübbe, Yvonne

AU - Correia, Telmo

AU - Schardl, Christopher L.

AU - Panaccione, Daniel G.

AU - Tudzynski, Paul

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

PY - 2005/6

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N2 - The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

AB - The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

KW - Chemical races

KW - Claviceps purpurea

KW - Ergocristine

KW - Ergot alkaloids

KW - Ergot fungus

KW - Ergotamine

KW - Gene cluster

KW - Nonribosomal peptide synthetases

KW - Phosphate effect

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M3 - Article

C2 - 15904941

AN - SCOPUS:20444414907

SN - 0031-9422

VL - 66

SP - 1312

EP - 1320

JO - Phytochemistry

JF - Phytochemistry

IS - 11 SPEC. ISS.

ER -

The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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