The interaction of divalent metal ions with tris buffer in dilute solution

David P. Hanlon; David S. Watt; Edward W. Westhead

doi:10.1016/0003-2697(66)90150-3

The interaction of divalent metal ions with tris buffer in dilute solution

David P. Hanlon
, David S. Watt
, Edward W. Westhead

Molecular and Cellular Biochemistry

Producción científica: Article › revisión exhaustiva

82 Citas (Scopus)

Resumen

The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg⁺⁺ and Cu⁺⁺ interact strongly with tris, and Co⁺⁺, Zn⁺⁺, Cd⁺⁺ much less so. The interaction of tris with Mg⁺⁺ and Mn⁺⁺ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

Idioma original	English
Páginas (desde-hasta)	225-233
Número de páginas	9
Publicación	Analytical Biochemistry
Volumen	16
N.º	2
DOI	https://doi.org/10.1016/0003-2697(66)90150-3
Estado	Published - ago 1966

Nota bibliográfica

Funding Information:
1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

Financiación

1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

Financiadores	Número del financiador
National Institute of Arthritis bolic Diseases	GM OS474

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(66)90150-3

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title = "The interaction of divalent metal ions with tris buffer in dilute solution",

abstract = "The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.",

author = "Hanlon, \{David P.\} and Watt, \{David S.\} and Westhead, \{Edward W.\}",

note = "Funding Information: 1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225",

year = "1966",

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doi = "10.1016/0003-2697(66)90150-3",

language = "English",

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T1 - The interaction of divalent metal ions with tris buffer in dilute solution

AU - Hanlon, David P.

AU - Watt, David S.

AU - Westhead, Edward W.

N1 - Funding Information: 1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

PY - 1966/8

Y1 - 1966/8

N2 - The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

AB - The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

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DO - 10.1016/0003-2697(66)90150-3

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

The interaction of divalent metal ions with tris buffer in dilute solution

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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