The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Thomas D. Webster; Robert C. Dickson

doi:10.1093/nar/16.16.8011

The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Thomas D. Webster
, Robert C. Dickson

Molecular and Cellular Biochemistry

Producción científica: Article › revisión exhaustiva

36 Citas (Scopus)

Resumen

The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

Idioma original	English
Páginas (desde-hasta)	8011-8028
Número de páginas	18
Publicación	Nucleic Acids Research
Volumen	16
N.º	16
DOI	https://doi.org/10.1093/nar/16.16.8011
Estado	Published - ago 25 1988

Nota bibliográfica

Funding Information:
ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.

Financiación

ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.

Financiadores	Número del financiador
Mason and Hanger Corporation
National Institutes of Health (NIH)
National Institute of General Medical Sciences	R01GM022749
U.S. Public Health Service

ASJC Scopus subject areas

Genetics

Acceder al documento

10.1093/nar/16.16.8011

Otros archivos y enlaces

Citar esto

@article{e47af6d56a424cd8ad3796e45b71a3f2,

title = "The organization and transcription of the galactose gene cluster of Kluyveromyces lactis",

abstract = "The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.",

author = "Webster, \{Thomas D.\} and Dickson, \{Robert C.\}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.",

year = "1988",

month = aug,

day = "25",

doi = "10.1093/nar/16.16.8011",

language = "English",

volume = "16",

pages = "8011--8028",

number = "16",

}

TY - JOUR

T1 - The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

AU - Webster, Thomas D.

AU - Dickson, Robert C.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.

PY - 1988/8/25

Y1 - 1988/8/25

N2 - The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

AB - The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

UR - https://www.scopus.com/pages/publications/0024297081

UR - https://www.scopus.com/pages/publications/0024297081#tab=citedBy

U2 - 10.1093/nar/16.16.8011

DO - 10.1093/nar/16.16.8011

M3 - Article

C2 - 3047676

AN - SCOPUS:0024297081

SN - 0305-1048

VL - 16

SP - 8011

EP - 8028

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 16

ER -

The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto