The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Barbara Téllez-Robledo; Concepcion Manzano; Angela Saez; Sara Navarro-Neila; Javier Silva-Navas; Laura de Lorenzo; Mary Paz González-García; René Toribio; Arthur G. Hunt; Roberto Baigorri; Ilda Casimiro; Siobhan M. Brady; M. Mar Castellano; J. Carlos del Pozo

doi:10.1111/tpj.14416

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Barbara Téllez-Robledo
, Concepcion Manzano
, Angela Saez
, Sara Navarro-Neila
, Javier Silva-Navas
, Laura de Lorenzo
, Mary Paz González-García
, René Toribio
, Arthur G. Hunt
, Roberto Baigorri
, Ilda Casimiro
, Siobhan M. Brady
, M. Mar Castellano
, J. Carlos del Pozo

Producción científica: Article › revisión exhaustiva

44 Citas (Scopus)

Resumen

Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

Idioma original	English
Páginas (desde-hasta)	1203-1219
Número de páginas	17
Publicación	Plant Journal
Volumen	99
N.º	6
DOI	https://doi.org/10.1111/tpj.14416
Estado	Published - sept 1 2019

Nota bibliográfica

Publisher Copyright:
© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd

Financiación

This research was supported by grants from the Spanish Government (BIO2017-82209-R and BIO2014-52091-R to J.C.P.) and by the "Severo Ochoa Program for Centres of Excellence in R&D” from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 (2017-2021)) to the CBGP. B.T. was supported by a predoctoral fellowship (BES-2012-054056) from MINECO (Spain). C.M. was supported by a Marie Skłodowska-Curie fellowship (Root Barriers 655406) from the European Commission. S.M.B is supported by an Howard Hughes Medical Institute (HHMI) Faculty Scholar Fellowship. We thank J.L. Micol and M.R. Ponce for mapping the fip1-2 mutation, and Jose Manuel Franco and Genomic4all for help with the RNAseq and biocomputational analyses. We also thank Mark Estelle for kindly providing us with the DR5:GFP seeds.

Financiadores	Número del financiador
Marie Skłodowska-Curie fellowship	655406
Spanish government	BIO2014-52091-R, BIO2017-82209-R
Howard Hughes Medical Institute
European Commission
Ministerio de Economía y Competitividad
Agencia Estatal de Investigación	BES-2012-054056, SEV-2016-0672
Agencia Estatal de Investigación

ASJC Scopus subject areas

Genetics
Plant Science
Cell Biology

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10.1111/tpj.14416

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Téllez-Robledo, B., Manzano, C., Saez, A., Navarro-Neila, S., Silva-Navas, J., de Lorenzo, L., González-García, M. P., Toribio, R., Hunt, A. G., Baigorri, R., Casimiro, I., Brady, S. M., Castellano, M. M., & del Pozo, J. C. (2019). The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses. Plant Journal, 99(6), 1203-1219. https://doi.org/10.1111/tpj.14416

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title = "The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses",

abstract = "Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.",

keywords = "Arabidopsis, FIP1, alternative polyadenylation, root development, stem cell maintenance, stress responses",

author = "Barbara T{\'e}llez-Robledo and Concepcion Manzano and Angela Saez and Sara Navarro-Neila and Javier Silva-Navas and \{de Lorenzo\}, Laura and Gonz{\'a}lez-Garc{\'i}a, \{Mary Paz\} and Ren{\'e} Toribio and Hunt, \{Arthur G.\} and Roberto Baigorri and Ilda Casimiro and Brady, \{Siobhan M.\} and Castellano, \{M. Mar\} and \{del Pozo\}, \{J. Carlos\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors The Plant Journal {\textcopyright} 2019 John Wiley \& Sons Ltd",

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Téllez-Robledo, B, Manzano, C, Saez, A, Navarro-Neila, S, Silva-Navas, J, de Lorenzo, L, González-García, MP, Toribio, R, Hunt, AG, Baigorri, R, Casimiro, I, Brady, SM, Castellano, MM & del Pozo, JC 2019, 'The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses', Plant Journal, vol. 99, n.º 6, pp. 1203-1219. https://doi.org/10.1111/tpj.14416

TY - JOUR

T1 - The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

AU - Téllez-Robledo, Barbara

AU - Manzano, Concepcion

AU - Saez, Angela

AU - Navarro-Neila, Sara

AU - Silva-Navas, Javier

AU - de Lorenzo, Laura

AU - González-García, Mary Paz

AU - Toribio, René

AU - Hunt, Arthur G.

AU - Baigorri, Roberto

AU - Casimiro, Ilda

AU - Brady, Siobhan M.

AU - Castellano, M. Mar

AU - del Pozo, J. Carlos

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

AB - Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

KW - Arabidopsis

KW - FIP1

KW - alternative polyadenylation

KW - root development

KW - stem cell maintenance

KW - stress responses

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M3 - Article

C2 - 31111599

AN - SCOPUS:85068147416

SN - 0960-7412

VL - 99

SP - 1203

EP - 1219

JO - Plant Journal

JF - Plant Journal

IS - 6

ER -

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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