The Same CCAAT Box-Binding Factor Binds to the Promoter of Two Coordinately Regulated Major Histocompatibility Complex Class II Genes

Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex Aβ CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex Eα CCAAT box. Although the two CCAAT boxes share the same 10-base core sequence, termed the Y box, their flanking sequences, known to be important for binding, are very different.