The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants

Qingshun Li; Carol Von Lanken; Jianjun Yang; Christopher B. Lawrence; Arthur G. Hunt

doi:10.1023/A:1006354010997

The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants

Qingshun Li
, Carol Von Lanken
, Jianjun Yang
, Christopher B. Lawrence
, Arthur G. Hunt

Plant and Soil Sciences

Producción científica: Article › revisión exhaustiva

15 Citas (Scopus)

Resumen

We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties - impairment of growth and induction of defense responses - indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.

Idioma original	English
Páginas (desde-hasta)	335-344
Número de páginas	10
Publicación	Plant Molecular Biology
Volumen	42
N.º	2
DOI	https://doi.org/10.1023/A:1006354010997
Estado	Published - 2000

Nota bibliográfica

Funding Information:
We are grateful to Maurice Swanson for the gift of antibody against the yeast PAB and Robert Houtz for providing anti-rbcL antisera. This work was supported in part by USDA-University of Kentucky Cooperative Agreement 58-43YK-7-0025 and USDA Competitive Grant 89-37262-4835. Q.L. and C.L. were supported by funds from the Kentucky Tobacco Research Board.

Financiación

We are grateful to Maurice Swanson for the gift of antibody against the yeast PAB and Robert Houtz for providing anti-rbcL antisera. This work was supported in part by USDA-University of Kentucky Cooperative Agreement 58-43YK-7-0025 and USDA Competitive Grant 89-37262-4835. Q.L. and C.L. were supported by funds from the Kentucky Tobacco Research Board.

Financiadores	Número del financiador
Kentucky Tobacco Research Board
USDA-University of Kentucky	58-43YK-7-0025
U.S. Department of Agriculture	89-37262-4835

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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title = "The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants",

abstract = "We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties - impairment of growth and induction of defense responses - indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.",

keywords = "Disease resistance, Lesion mimic, Poly(A)-binding proteins, Posttranscriptional control, SAR",

author = "Qingshun Li and \{Von Lanken\}, Carol and Jianjun Yang and Lawrence, \{Christopher B.\} and Hunt, \{Arthur G.\}",

note = "Funding Information: We are grateful to Maurice Swanson for the gift of antibody against the yeast PAB and Robert Houtz for providing anti-rbcL antisera. This work was supported in part by USDA-University of Kentucky Cooperative Agreement 58-43YK-7-0025 and USDA Competitive Grant 89-37262-4835. Q.L. and C.L. were supported by funds from the Kentucky Tobacco Research Board.",

year = "2000",

doi = "10.1023/A:1006354010997",

language = "English",

volume = "42",

pages = "335--344",

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TY - JOUR

T1 - The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants

AU - Li, Qingshun

AU - Von Lanken, Carol

AU - Yang, Jianjun

AU - Lawrence, Christopher B.

AU - Hunt, Arthur G.

N1 - Funding Information: We are grateful to Maurice Swanson for the gift of antibody against the yeast PAB and Robert Houtz for providing anti-rbcL antisera. This work was supported in part by USDA-University of Kentucky Cooperative Agreement 58-43YK-7-0025 and USDA Competitive Grant 89-37262-4835. Q.L. and C.L. were supported by funds from the Kentucky Tobacco Research Board.

PY - 2000

Y1 - 2000

N2 - We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties - impairment of growth and induction of defense responses - indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.

AB - We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties - impairment of growth and induction of defense responses - indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.

KW - Disease resistance

KW - Lesion mimic

KW - Poly(A)-binding proteins

KW - Posttranscriptional control

KW - SAR

UR - https://www.scopus.com/pages/publications/0034049618

UR - https://www.scopus.com/pages/publications/0034049618#tab=citedBy

U2 - 10.1023/A:1006354010997

DO - 10.1023/A:1006354010997

M3 - Article

C2 - 10794533

AN - SCOPUS:0034049618

SN - 0167-4412

VL - 42

SP - 335

EP - 344

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 2

ER -

The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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