Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Hiroko P. Indo; Hiromu Ito; Keiichi Nakagawa; Luksana Chaiswing; Hideyuki J. Majima

doi:10.1016/j.abb.2021.108853

Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Hiroko P. Indo, Hiromu Ito, Keiichi Nakagawa, Luksana Chaiswing, Hideyuki J. Majima

Toxicology and Cancer Biology

Producción científica: Article › revisión exhaustiva

3 Citas (Scopus)

Resumen

Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

Idioma original	English
Número de artículo	108853
Publicación	Archives of Biochemistry and Biophysics
Volumen	703
DOI	https://doi.org/10.1016/j.abb.2021.108853
Estado	Published - may 30 2021

Nota bibliográfica

Publisher Copyright:
© 2021 Elsevier Inc.

Financiación

The HSP47 vector was a kind gift from Professor Kazuhiro Nagata of Kyoto Sangyo University. The authors would like to express sincere appreciation for the gift. The authors gratefully thank Drs Shosei Kishida and Shigeaki Suenaga of Kagoshima University, Drs. Noriaki Ishioka and Sachiko Yano of Japan Aerospace Exploration Agency (JAXA), and Drs. Daret K St. Clair and William St. Clair of University of Kentucky for encouragement to complete this study. We thank Mr. Shigeki Saegusa of the University of Tokyo Hospital for his help and guidance on electron irradiation.

Financiadores	Número del financiador
Drs Shosei Kishida and Shigeaki Suenaga of Kagoshima University
Kyoto Sangyo University
Japan Aerospace Exploration Agency

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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@article{130b071f972f44eeb3aa0593ee0b02be,

title = "Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation",

abstract = "Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.",

keywords = "Electron and X-ray irradiation, Farnesylation, HSP47, Mitochondria, Myristoylation, Reactive oxygen species",

author = "Indo, \{Hiroko P.\} and Hiromu Ito and Keiichi Nakagawa and Luksana Chaiswing and Majima, \{Hideyuki J.\}",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = may,

day = "30",

doi = "10.1016/j.abb.2021.108853",

language = "English",

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T1 - Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

AU - Indo, Hiroko P.

AU - Ito, Hiromu

AU - Nakagawa, Keiichi

AU - Chaiswing, Luksana

AU - Majima, Hideyuki J.

PY - 2021/5/30

Y1 - 2021/5/30

N2 - Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

AB - Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

KW - Electron and X-ray irradiation

KW - Farnesylation

KW - HSP47

KW - Mitochondria

KW - Myristoylation

KW - Reactive oxygen species

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M3 - Article

C2 - 33811847

AN - SCOPUS:85104139694

SN - 0003-9861

VL - 703

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

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Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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