Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions

Xin Guo; Birgit Schilling; Malin Young; Matyas Medzihradszky; Irwin D. Kuntz; R. Kip Guy; Bradford W. Gibson

Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions

Xin Guo, Birgit Schilling, Malin Young, Matyas Medzihradszky, Irwin D. Kuntz, R. Kip Guy, Bradford W. Gibson

Producción científica: Paper › revisión exhaustiva

4 Citas (Scopus)

Resumen

The determination of protein structure and protein-protein interactions using homobifunctional crosslinking reagents with normal and N-15 labeled proteins was discussed. Recombinant CMP-NeuAc synthetase and HIV-integrase were prepared from normal and N-15 enriched media. In the case of crosslinked peptides originating from protein-protein interactions, some gain was obtained from a pattern recognition point of view, since these peptide types were observed as quartets. The identification of crosslinked peptides was confirmed by electrospray ionization (ESI)-mass spectrometry (MS)/MS using a quadrupole time-of-flight (TOF) instrument or MALDI-PSD.

Idioma original	English
Páginas	249-250
Número de páginas	2
Estado	Published - 2002
Evento	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duración: jun 2 2002 → jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
País/Territorio	United States
Ciudad	Orlando, FL
Período	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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Guo, X., Schilling, B., Young, M., Medzihradszky, M., Kuntz, I. D., Guy, R. K., & Gibson, B. W. (2002). Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions. 249-250. Papel presentado en Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

@conference{e5df290a4c25439dad9e7c36e640130d,

title = "Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions",

abstract = "The determination of protein structure and protein-protein interactions using homobifunctional crosslinking reagents with normal and N-15 labeled proteins was discussed. Recombinant CMP-NeuAc synthetase and HIV-integrase were prepared from normal and N-15 enriched media. In the case of crosslinked peptides originating from protein-protein interactions, some gain was obtained from a pattern recognition point of view, since these peptide types were observed as quartets. The identification of crosslinked peptides was confirmed by electrospray ionization (ESI)-mass spectrometry (MS)/MS using a quadrupole time-of-flight (TOF) instrument or MALDI-PSD.",

author = "Xin Guo and Birgit Schilling and Malin Young and Matyas Medzihradszky and Kuntz, \{Irwin D.\} and Guy, \{R. Kip\} and Gibson, \{Bradford W.\}",

year = "2002",

language = "English",

pages = "249--250",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Guo, X, Schilling, B, Young, M, Medzihradszky, M, Kuntz, ID, Guy, RK & Gibson, BW 2002, 'Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions', Papel presentado en Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 249-250.

Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions. / Guo, Xin; Schilling, Birgit; Young, Malin et al.
2002. 249-250 Papel presentado en Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Producción científica: Paper › revisión exhaustiva

TY - CONF

T1 - Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions

AU - Guo, Xin

AU - Schilling, Birgit

AU - Young, Malin

AU - Medzihradszky, Matyas

AU - Kuntz, Irwin D.

AU - Guy, R. Kip

AU - Gibson, Bradford W.

PY - 2002

Y1 - 2002

N2 - The determination of protein structure and protein-protein interactions using homobifunctional crosslinking reagents with normal and N-15 labeled proteins was discussed. Recombinant CMP-NeuAc synthetase and HIV-integrase were prepared from normal and N-15 enriched media. In the case of crosslinked peptides originating from protein-protein interactions, some gain was obtained from a pattern recognition point of view, since these peptide types were observed as quartets. The identification of crosslinked peptides was confirmed by electrospray ionization (ESI)-mass spectrometry (MS)/MS using a quadrupole time-of-flight (TOF) instrument or MALDI-PSD.

AB - The determination of protein structure and protein-protein interactions using homobifunctional crosslinking reagents with normal and N-15 labeled proteins was discussed. Recombinant CMP-NeuAc synthetase and HIV-integrase were prepared from normal and N-15 enriched media. In the case of crosslinked peptides originating from protein-protein interactions, some gain was obtained from a pattern recognition point of view, since these peptide types were observed as quartets. The identification of crosslinked peptides was confirmed by electrospray ionization (ESI)-mass spectrometry (MS)/MS using a quadrupole time-of-flight (TOF) instrument or MALDI-PSD.

UR - https://www.scopus.com/pages/publications/0042747320

UR - https://www.scopus.com/inward/citedby.url?scp=0042747320&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:0042747320

SP - 249

EP - 250

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Using homobifunctional crosslinking reagents with normal and N-15 labeled proteins for the determination of protein tertiary structure and protein-protein interactions

Resumen

Conference

ASJC Scopus subject areas

ODS de las Naciones Unidas

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