Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses

Stephen C. Mayer; Jennifer K. Stevenson; Siva Gandhapudi; Jerold G. Woodward

doi:10.1016/j.jim.2025.114007

Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses

Stephen C. Mayer
, Jennifer K. Stevenson
, Siva Gandhapudi
, Jerold G. Woodward

Producción científica: Article › revisión exhaustiva

Resumen

During the COVID-19 Pandemic in 2020, the need for highly-specific, wide-spread, and rapid serological testing surged. In this study, we tested the utility of the Volumetric Absorptive Microsampling (VAMS) using Mitra home sampling kits by comparing SARS CoV2 spike antibody levels from serum or Mitra dried blood samples from the same individuals with or without prior COVID 19 infection. We showed a very strong correlation between venous blood collection and capillary VAMS for the detection of anti-SARS CoV2 IgGs using a sensitive, semi-quantitative ELISA protocol. Furthermore, we found that the antibody levels detected from the Mitra blood samples were stable at room temperature for several months. This study demonstrates the utility of using at-home, patient-centric testing to enhance the sero-surveillance methods and large scale community testing for viral tracking, monitoring and vaccine studies.

Idioma original	English
Número de artículo	114007
Publicación	Journal of Immunological Methods
Volumen	545
DOI	https://doi.org/10.1016/j.jim.2025.114007
Estado	Published - dic 2025

Nota bibliográfica

Publisher Copyright:
Copyright © 2025. Published by Elsevier B.V.

Financiación

This study was funded by the National Center for Research Resources and the National Center for Advancing Translational Sciences, the National Institutes of Health through Grant UL1TR001998 to the Center for Clinical and Translational Science, pilot Funding to J.G.W. and S.K.G. Support was also provided through the Office of the Vice President for Research CURE program at the University of Kentucky.

Financiadores	Número del financiador
National Center for Advancing Translational Sciences (NCATS)
National Center for Research Resources
University of Kentucky
National Institutes of Health (NIH)	UL1TR001998

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses",

abstract = "During the COVID-19 Pandemic in 2020, the need for highly-specific, wide-spread, and rapid serological testing surged. In this study, we tested the utility of the Volumetric Absorptive Microsampling (VAMS) using Mitra home sampling kits by comparing SARS CoV2 spike antibody levels from serum or Mitra dried blood samples from the same individuals with or without prior COVID 19 infection. We showed a very strong correlation between venous blood collection and capillary VAMS for the detection of anti-SARS CoV2 IgGs using a sensitive, semi-quantitative ELISA protocol. Furthermore, we found that the antibody levels detected from the Mitra blood samples were stable at room temperature for several months. This study demonstrates the utility of using at-home, patient-centric testing to enhance the sero-surveillance methods and large scale community testing for viral tracking, monitoring and vaccine studies.",

keywords = "ELISA, SARS CoV2, Sero-surveillance, VAMS",

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doi = "10.1016/j.jim.2025.114007",

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AU - Mayer, Stephen C.

AU - Stevenson, Jennifer K.

AU - Gandhapudi, Siva

AU - Woodward, Jerold G.

PY - 2025/12

Y1 - 2025/12

N2 - During the COVID-19 Pandemic in 2020, the need for highly-specific, wide-spread, and rapid serological testing surged. In this study, we tested the utility of the Volumetric Absorptive Microsampling (VAMS) using Mitra home sampling kits by comparing SARS CoV2 spike antibody levels from serum or Mitra dried blood samples from the same individuals with or without prior COVID 19 infection. We showed a very strong correlation between venous blood collection and capillary VAMS for the detection of anti-SARS CoV2 IgGs using a sensitive, semi-quantitative ELISA protocol. Furthermore, we found that the antibody levels detected from the Mitra blood samples were stable at room temperature for several months. This study demonstrates the utility of using at-home, patient-centric testing to enhance the sero-surveillance methods and large scale community testing for viral tracking, monitoring and vaccine studies.

AB - During the COVID-19 Pandemic in 2020, the need for highly-specific, wide-spread, and rapid serological testing surged. In this study, we tested the utility of the Volumetric Absorptive Microsampling (VAMS) using Mitra home sampling kits by comparing SARS CoV2 spike antibody levels from serum or Mitra dried blood samples from the same individuals with or without prior COVID 19 infection. We showed a very strong correlation between venous blood collection and capillary VAMS for the detection of anti-SARS CoV2 IgGs using a sensitive, semi-quantitative ELISA protocol. Furthermore, we found that the antibody levels detected from the Mitra blood samples were stable at room temperature for several months. This study demonstrates the utility of using at-home, patient-centric testing to enhance the sero-surveillance methods and large scale community testing for viral tracking, monitoring and vaccine studies.

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Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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